Showing posts with label build strength. Show all posts
Showing posts with label build strength. Show all posts

Wednesday, November 4, 2015

Milk Fat Globule Membranes Power Up Strength Gains by Improving the Wiring of Subjects' Muscles | Plus: Its Proven Health, EPO & Immune-Boosting Effects Won't Hurt Either

At the moment it is by no means clear which type of athlete / gymrat would benefit from milk fat globule membrane supplementation and which wouldn't. It's after all well possible that the effects Soga et al. observed in rookies wouldn't occur in adv. trainees or professional athletes.
After the study on nucleotide supplements the Satako Soga's latest paper on the dietary supplementation with milk fat globule membrane is #2 on the list of studies with promising new ergogenics in less than a month. In view of the fact that the last year has been devoid of any true innovations, this is a welcome diversion from writing about whey (or other protein powders), creatine, beta alanine and baking soda, as well as the occasional study on HMB for me.

In spite of the fact that this is a follow up on previous rodent studies from the same researchers, I must warn you not to to expect too much of MFGMs, yet. More data and studies with more realistic resistance training + supplementation protocols from independent labs are warranted before MFGMs may eventually appear next to the previously mentioned top dogs on a list of "waredownloadsoft Recommended Supplements".
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But hey, let's keep the skepticism for the bottom line and take a look at what the scientists from the Kao Corporation did with their fourteen Japanese subjects. The men, who were aged 31–48 years and did not participate in regular strength training before the study, took 1 g of the  structural membranes of milk which are usually covering the triglyceride globules that are dispersed as emulsified bodies in milk or 1g of an identically looking whole milk powder as a placebo in tablet form for 4-weeks (composition see Table 1).
Table 1: Composition of MFGM and whole milk powder (Soga. 2015)
"On the exercise training enforcement days [i.e. the days on which the subjects were required to train], the subjects were instructed to take the tablet within 1 h before training. On the other days, the subjects were instructed to consume the tablet at the time of their choice during their daily routines.
The MFGM was prepared from buttermilk by filtering and centrifugation. The MFGM and whole milk powder compositions were analyzed at Japan Food Research Laboratories (Tokyo, Japan)" (Soga. 2015).
The exercise routine the subjects from both groups had to follow consisted of two workouts per week. The workouts had to be performed on nonconsecutive days for 4 weeks using StrengthErgo 240 stationary cycling exercise machines (Mitsubishi Electric Corporation, Tokyo, Japan). On these machines, the subjects completed 3 sets of 15 % maximal voluntary contraction (MVC) cycle exercises for 60 s and 7 sets of 20 % MVC cycle exercises for 40 s at 50 rpm.
Preliminary rodent studies show that sign. increases in swimming time to exhaustion occur with as little as the equivalent of 1g/day of MFMG (Haramizu. 2014a) - The dose-dependency of the effects suggest that it may be worth taking more than 1g/day for humans, too.
What's the optimal dosage? It is way too early to answer this question with confidence, but the dosage in Soga's study was selected based on the results of previous rodent studies, where the equivalent of the 1 g the subjects consumed in the study at hand turned out to be the minimal effective dosage. In view of the fact that you will find the same amount of MFGM in just 600 ml of full fat milk, I am yet inclined to believe that it may be worth checking if dose-escalations would not yield even better and still side-effect free increases in strength gains and/or increases in endurance performance and fatty acid oxidation as they were observed in previous rodent studies (Haramizu. 2014a). Furthermore, preliminary data from a 4-week safety evaluation by Hari et al. (2015) shows that even 6.5 g/day are without side-effects in healthy humans.
Physical function tests and surface electromyography (EMG) were conducted at baseline and at the end of the study period. In addition, some hemotological parameters were tested of which - surprise - the data in Figure 1 tells you that MFGMs have a small, but statistically significant "EPO-effect" and are thus capable of raising both the red blood cell and hemoglobin content - an effect that has been observed in rodents too (Haramizu. 2014b) and may be ascribed to the incorporation of MFGM phospholipids into red blood cells which would in turn be less vulnerable to exercise-related or other stressors. Whatever the reason for these changes may be, they are certainly in line with the previously mentioned (see red box) increase in fatty acid oxidation during endurance exercise that may be facilitated by an RBC and hemoglogin related increase in oxygen transport.
Figure 1: The significant increase in red blood cell and hemoglogin content could explain the previously observed increases in endurance performance and fatty acid oxidation in Haramizu et al. (2014a). The buffered decrease in white blood cell may not be statistically significant, but may still indicate beneficial effects on the immune system (Soga. 2015).
Both the increase in RBC and hemoglobin, as well as the buffering of the albeit non-significant decline in white blood cells in the placebo group, which may be a response to the exercise-induced stress and evidence of an immune-boosting effect of MFGM supplementation during any potentially exercise induced immune suppression, do yet need further investigation before they can be explained mechanistically.

No further research is needed, though, to state that all other blood markers. These included AST + ALT (liver health), glucose and triglycerides + cholesterol, neither of which was affected by the supplement. The latter cannot be said of the increase in leg extension strength before and after the intervention and the EMG activity which showed highly significant inter-group differences.
Do I even have to supplement? I mean, I can just drink 600ml of full fat milk, right? I don't doubt that you can do just that. The problem is that the milk you can drink will probably be pasturized - a process of which Cano-Ruiz et al. (1997) have shown that it will induce significant and potentially functionally relevant differences in the composition of the milk fat globule membrane. Whether the supplement that was used at the study at hand was made during the production of buttermilk before or after pasteurization is not clear. If we assume it was made after pasteurization, though, you could in fact hope for similar results from pasteurized whole milk. Otherwise, the milk would have to be raw (even if it is, though it cannot be guaranteed that the same amount of MFGMs from milk will have identical effects).
In fact, the differences in Figure 2 are pronounced enough to ask the question: Were they induced by the supplementation alone or did the pathetic training regimen have an effect here, as well? As I am going to point out in the bottom line one paragraph below, we will need studies with more realistic training programs to answer this question in a way that's relevant for trainees like you and me. In the mean time, however, there is no debating "that the daily intake of 1 g MFGM combined with regular, twice weekly exercise improved skeletal muscle strength (leg extension) in middle-aged adults, despite a lack of change in muscle mass" (Soga. 2015).
Figure 2: The MFGM supplementation lead to significant increases in leg extension strength, of which the increase in EMG activity (likewise sign.) suggests that they were mediated by neuronal changes (Soga. 2015).
The same goes for the increase in RMS of surface EMG, which indicates that the dietary provision of MFGM increased the average (RMS = root mean square) motor unit activity during muscle contraction and may thus mechanistically explain why the subjects got stronger even though they didn't make substantial gains in muscle mass: The supplement helped them to "make the most" of the muscle they had. This hypothesis is in line with results from rodent studies which
"[...] revealed that dietary MFGM combined with regular exercise improved muscle strength in adult mice primarily by stimulating the pathway involving “nervous system development” in the skeletal muscle (Haramizu. 2014b). This pathway includes functional annotations such as formation of synapses, growth of neurites, or development of NMJ. Dietary MFGM combined with exercise increased skeletal muscle expression of docking protein-7 (Dok-7) and muscle-specific receptor tyrosine kinase in mice, both of which play a critical role in NMJ [neuromuscular junction] formation. Defects in NMJ function causes muscle weakness in neuromuscular disorders, and Dok-7 gene therapy improves NMJ formation and rescues the motor activity" (Soga. 2015).
And still, even at the risk that I may sound like a broken record, I have to repeat that without future research it is impossible to tell (a) whether the assumptions we've made about the increase in red blood cell count are accurate and, maybe even more importantly, (b) who, i.e. which type of athlete, will benefit most from using MFGM supplements.
MFGMs can do much more! It's not like this was the first study on MFGMs. It's just the first study on exercise performance in humans. In rodents performance incre- ments (see red box), as well as increases in RBC and EMG have long been established. In older women MFGMs have been shown to improve or fully reverse frailty (Kim. 2015). As hinted at in the conclusion, the addition of MFGMs to infant formulas will also narrow the gap in cognitive development and lipid composition (+future VCD risk) between breastfed and formula-fed infants (Timby. 2014a,b).

Other purported benefits include anti-cancer effects (Lemonnier. 2003) stress protection, the potential to reduce the risk of Alzheimer's (Spitsberg. 2005) and increase gastrointestinal intergrity (Snow. 2010), and beneficial effects on blood lipids (Noh. 2004) and cardio-vascular health where some researchers expect beneficial, while others fear detrimental effects (Riccio. 2004; Spitsberg. 2005; Singh. 2006). Since most of these effects have been observed in vitro or in rodent studies, it is yet by no means clear which of them would occur in response to MFGM supplementation.
Bottom line: As I already pointed out in the introduction, it is way too early to celebrate MFGMs as "the next big thing" or the supplemental reincarnation of the "a gallon of milk per day" protocol which would have provided its followers a highly significant 6g+ dose of MFGM on a daily basis.

The reason(s) we need further research are simple. Firstly, we need independent validation of the results that were generated by scientists who work for a company that produces milk fat globule membranes. Secondly, studies in trained individuals in whom the purportedly mechanistically involved improvements in the neuronal wiring of the muscle may be significantly less pronounced, as well as studies involving more realistic resistance training and endurance training protocols to confirm the beneficial effects that were observed in this study and the rodent study by Haramizu et al. which found significant increases in maximal endurence (see red box) are required to be able to tell for whom MFGM supplements will be beneficial. And third- and lastly, experiments with different dosing protocols (timing and amount) are necessary to determine the optimal dosing schemes - preferably for different types of exercises.

With that being said, MFGMs are still one of the more promising newcomers, lately. Their effects are after all not restricted to performance enhancing in the narrow(er) sense, but extend into the realms of general health and fitness (see box on the right hand side of this bottom line). In conjunction with their potential use as additives to baby formula, where they have already been shown to improve risk markers of future cardiometabolic disease and the babies' cognitive development to an extent that marginalizes the benefits of breast-feeding in these areas, make them one of the hottest candidates on my personal "still insufficient evidence, but promising"-list of supplement to keep an eye on | Comment on Facebook!
References:
  • Cano-Ruiz, M. E., and R. L. Richter. "Effect of homogenization pressure on the milk fat globule membrane proteins." Journal of Dairy Science 80.11 (1997): 2732-2739.
  • Haramizu, Satoshi, et al. "Dietary milk fat globule membrane improves endurance capacity in mice." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 307.8 (2014): R1009-R1017.
  • Haramizu, Satoshi, et al. "Habitual exercise plus dietary supplementation with milk fat globule membrane improves muscle function deficits via neuromuscular development in senescence-accelerated mice." SpringerPlus 3 (2014): 339.
  • Kim, Hunkyung, et al. "Effects of Exercise and Milk Fat Globule Membrane (MFGM) Supplementation on Body Composition, Physical Function, and Hematological Parameters in Community-Dwelling Frail Japanese Women: A Randomized Double Blind, Placebo-Controlled, Follow-Up Trial." PloS one 6.10.2 (2015): e0116256.
  • Lemonnier, Lori A., et al. "Sphingomyelin in the suppression of colon tumors: prevention versus intervention." Archives of Biochemistry and Biophysics 419.2 (2003): 129-138.
  • Riccio, Paolo. "The proteins of the milk fat globule membrane in the balance." Trends in food science & technology 15.9 (2004): 458-461.
  • Singh, Harjinder. "The milk fat globule membrane—A biophysical system for food applications." Current Opinion in Colloid & Interface Science 11.2 (2006): 154-163.
  • Snow, D. R., et al. "Membrane-rich milk fat diet provides protection against gastrointestinal leakiness in mice treated with lipopolysaccharide." Journal of dairy science 94.5 (2011): 2201-2212.
  • Soga, Satoko, Noriyasu Ota, and Akira Shimotoyodome. "Dietary milk fat globule membrane supplementation combined with regular exercise improves skeletal muscle strength in healthy adults: a randomized double-blind, placebo-controlled, crossover trial." Nutrition Journal 14.1 (2015): 85.
  • Spitsberg, V. L. "Invited review: Bovine milk fat globule membrane as a potential nutraceutical." Journal of dairy science 88.7 (2005): 2289-2294.
  • Timby, Niklas, et al. "Neurodevelopment, nutrition, and growth until 12 mo of age in infants fed a low-energy, low-protein formula supplemented with bovine milk fat globule membranes: a randomized controlled trial." The American journal of clinical nutrition 99.4 (2014a): 860-868.
  • Timby, Niklas, et al. "Cardiovascular risk markers until 12 mo of age in infants fed a formula supplemented with bovine milk fat globule membranes." Pediatric research 76.4 (2014b): 394-400.
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Training "On Cycle", Done Right - Women See Much Better Results When Periodization is in Line W/ Menstrual Cycle

Yes, I could have exploited the ambiguity and called this article "Training 'On Cycle', Done Right - Women See Much Better Results When Periodization is in Line W/ Their Period", but let's be honest: This is a science website and that's neither scientific, nor actually funny, is it?
As a man, I have to admit to be at best well-read, yet not experienced in all things "menstrual cycle". So, while I do only know from the (very different things) I've heard from (ex-)girl friends about how they feel during the different phases, I do know that the hormonal differences in the luteal phase, with high levels of progesterone and estrogen, and the follicular phase with low progesterone and eventually increasing estrogen levels are pronounced enough to cause much more than just mood disturbances.

For many trainers, however, the estrous cycle is still a closed book. "Can you train, or not!?" Especially male trainers are not just insensitive when they ask their protégées this question, they may also be missing out on a chance to maximize their clients' training progress. That's at least what a recent 4-months study from the Umea University in Sweden (Wikström-Frisén. 2015) suggests.
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According to Wikström-Frisén and colleagues, "high frequency periodized leg resistance training during the first two weeks of the menstrual cycle is more beneficial to optimize training, than the last two weeks" (ibid. 2015). Now, "beneficial" is obviously a very loosely defined term. When I am telling you, though, that power, strength and lean body mass gains all benefited from the right timing of the workouts (in the first two weeks of the estrous cycle), I will hopefully have every women's and every trainers' attention (even though, I guess I will lose even more of the male bros, now).
Figure 1: Relative changes in lean mass (DXA data), measures power and strength (torque) in 59 trained women in response two weeks of frequent leg-training in the first or second two weeks of their estrous cycle (Wikström-Frisén. 2015).
While all the aforementioned increases in the women who trained in the first two weeks of their estrous cycle were statistically significant (for all, but the quad torque test | +4.4% the statistical significance also survived the Benferroni corrections), the women in the group for whom the periodization scheme had a focus on the second two weeks of their menstrual cycle, saw no significant changes in lean mass and power and a significant reduction in quad strength (see Figure 1). Since the latter lost its statistical power, after Benferroni corrections, though, one could say that the changes the Swedish researchers observed in the 2nd weeks group were practically meaningless.
What about women on oral contraception? The scientists recruited 32 young women on oral contraceptives and 27 women who didn't use oral contraceptives and a re-analysis of the data in Figure 1 didn't show significant inter-group differences between the two groups. In other words, the data in Figure 1 and thus the main findings are relevant for "all" resistance training young women, irrespective of whether they're taking contraceptives, or not. The only difference is that you go by the contraceptive (CC), instead of the estrous cycle and place the high frequency training period in the first, not the last two weeks of the CC cycle.
"Meaningless changes", however, are not meaningless results. In fact, the exact opposite is the case. These results tell trainers and female trainees, alike, that abandoning their protégées / their own
  • regular non-periodized training, i.e. three leg training workouts per week that consisted of leg presses and leg curls (3x sets @ 8-10RM, 1-2 minutes rest between sets; progressive increase of weight by 2-10% whenever the subjects could perform 3x10 reps with a given weight) 
for 4-months and switching to a periodized 2-week high- vs. 2-week low-frequency training, where they would perform the same 48 workouts in either
  • high-frequency first cycles, i.e. 5 workouts per week in the first two weeks, 1 workout per week for the last two weeks of each menstrual / contraceptive cycle, or
  • high-frequency last cycles, i.e. 1 workout per week in the first two weeks, 4 workouts per week for the last two weeks of each menstrual / contraceptive cycle,
would have beneficial effects on their progress only if they increase the frequency during the early phase of the cycle. 
Figure 2: Comparison of the relative changes in the periodization group (high frequency in the first two weeks of the menstrual / CC cycle) vs. control group (three workouts per week for 4 months | Wikström-Frisén. 2015).
Ok, if you compare the periodization group to the control group which kept the regular "three workouts per week"-frequency (see Figure 2, green bars) was maintained, the "advantages" of periodizing "correctly" are not as pronounced as they are in comparison to doing it the "wrong" way. Even though, only the hamstrings appear to benefit to a large extent from periodization, though, benefits exist.

What's even more important, though, is the simple, but really important revelation (or for the few of you who have read about this before e.g. in Reis et al. (1995) "confirmation") that a woman's menstrual and similarly her contraceptive cycle must be aligned to her training schedule. Obviously, the implications will have to be further explored in future studies. Studies, of which I hope, that they will be using smarter periodization schemes which acknowledge that training only once a week is simply not enough... ;-)
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Bottom line: Before I try to put things into perspective, I should mention that the participants of the study who were recruited at local gyms, were not jut healthy, non-smokers and had regular menses, they were also experienced trainees. All of them had been doing leg presses and leg-curls for several months - in fact, on average for 3.5 years. Against that background, even non-statistical significant inter-group differences as they were observed between the periodization (5 per week, 1 per week) and the control group (3 per week) may be practically relevant, because they may help experienced trainees to break through plateaus.

With that being said, I personally think of this study as one study in a series of studies that will hopefully elucidate how women can adapt their training regimen to the repetitive changes in the hormonal milieu of their bodies.

If we are honest with ourselves, the fact that Wikström-Frisén's results come as a surprise to most of us is only further evidence of how wantonly exercise scientists and trainers, alike, have hitherto neglected the peculiarities of the female physiology and endocrinology | Comment on Facebook!
References:
  • Reis, E., U. Frick, and D. Schmidtbleicher. "Frequency variations of strength training sessions triggered by the phases of the menstrual cycle." International journal of sports medicine 16.8 (1995): 545-550.
  • Wikström-Frisén, L., C. J. Boraxbekk, and K. Henriksson-Larsén. "Effects on power, strength and lean body mass of menstrual/oral contraceptive cycle based resistance training." The Journal of sports medicine and physical fitness (2015).
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Resting 3 vs. 1 Min. Between Sets Pays Off: Greater Size + Strength Gains - Probably Mediated by 15% Higher Volume

Resting long enough to maximize your training volume could be the key to success, i.e. strength and size gains.
If you have been following the various affords to ascribe differences in strength and, even more so, size-increases to a specific training variable, you will remember that the only promising parameters that appear to be supported by more than the literal "outlier study" are training load and volume.

Of these, the former is pretty much uncontested. The latter, however, is still questioned by a camp of inconvincible skep- tics, who simply ignore the fact that there's ample evidence that "[h]igher-volume, multiple-set protocols have consistent- ly proven superior over single set protocols with respect to increased muscle hypertrophy" (Schoenfeld. 2010).
It would be interesting to see if rest periods should also be periodized!

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What still isn't clear, though, is the role of other training parameters, such as the time you take to recover between multiple sets and exercises aka the "rest intervals". As Schoenfeld et al. point out in the introduction to their most recent study, "several studies have investigated the effects of varying rest interval length on muscular adaptations," (Schoenfeld. 2015) albeit with contradictory results: While Ahtiainen et al (2005) were unable to find a significant inter-group size or strength difference in well-trained subjects (6.6 +/- 2.8 years of continuous strength training) who rested 2 minutes compared to those who rested only 5 minute in response to their 21-week training intervention, Buresh et al (2005) reported more recently that significantly greater size increases of the arms and a trend for greater muscle hypertrophy in the legs in young, albeit untrained subjects who rested for 2.5 minutes instead of just one.
Figure 1: Previous studies found "conflicting" evidence. While Ahtiainen et al. found no effects of 2 vs. 5 minutes in trained, Buresh et al. found effects of 2 vs. 1 minutes rest in untrained subjects. With different subjects, different workouts and most importantly different rest times that were compared it is yet not exactly right to say that the studies contradict each other.
Now, obviously, the ostensible "contradiction" I alluded to in the previous paragraph does eventually not exist. With trained vs. untrained subjects, different workout protocols and most importantly different rest intervals (1 vs. 2 minutes and 2 vs. 5 minutes) the studies by Ahtiainen and Buresh cannot really contradict each other. The same must be said of an even more recent study by Villanueva et al. (2014) the surprising findings of which, i.e. "longer rest periods compromise the gains of older trainees", I've discussed last year, already.
What about the lack of different increases in strength endurance? I have to admit that I do not discuss this finding of the study in detail. While one would expect that shorter rest intervals would produce greater strength endurance adaptations, the researchers observed the opposite, an - albeit non-significantly larger increase in strength endurance in the 3-minute-rest group that correlated with the increase in 1RM strength. Further studies will have to show what the underlying mechanism of this counter-intuitive observation is and whether it may be muscle specific, i.e. occur only in the upper, but not in the lower body.
Eventually, however, this does not change that there is, as Schoenfeld et al. write that "a need for more research to provide greater clarity on the topic" (Schoenfeld. 2015). A "clarity" Schoenfeld et al. sought to find with a study that "used current rest interval recommendations for hypertrophy and strength of 1 versus 3 minutes, respectively, and employed validated measures to directly assess site-specific changes in muscle thickness" (ibid). In that, the researchers speculated that ...
"[c]onsistent with generally accepted guidelines on the topic (Willardson. 2006), we hypothesized that short rest intervals would produce greater increases in muscle growth and local muscle endurance while long rest intervals would result in superior strength increases" (Schoenfeld. 2015).
As you will know if you didn't miss the headline of this waredownloadsoft article, this hypothesis was only partly validated. The data in Figure 2 confirms that the subjects, "experienced lifters (defined as consistently lifting weights for a minimum of 6 months and a back squat / body weight ratio ≥ 1.0)" (Schoenfeld. 2015), gained significantly more strength, when they rested 3 versus just 1 minute between the 3 sets of their three weekly workouts (Figure 2 does also tell you that the strength endurance increases were identical in both groups).
Figure 2: Changes in markers of strength and strength endurance; * denotes significant pre- vs. post difference, # denotes significant inter-group difference (here in favor of long(er) rest periods | Schoenfeld. 2015).
What was Schoenfeld et al. did not find, however, were increased size gains in the short-rest period group whose 24 workouts that were performed on non-consecutive days over the course of the 8-week study period, were otherwise identical with those of the long-rest period group and comprised a total of 7 exercises for all major body parts, namely...
  • three leg exercises, i.e. barbell back squats, plate-loaded leg presses, and plate-loaded leg extensions), 
  • two exercises for the anterior torso muscles, i.e. flat barbell presses and seated barbell military presses, and 
  • two exercises for the posterior torso muscles, i.e. wide-grip plate-loaded lateral pulldowns, and plate-loaded seated cable rows
This is a highly significant result even for you who is - according to an older waredownloadsoft Poll - probably training according to a split regimen, albeit most likely with very similar exercises. What may be different from the some, but obviously *smile* not your workout though, is that the supervision by members of the research team ensured that the subjects stuck to the prescribed cadence of 1 second for the concentric and "approximately 2 seconds" (ibid.) for the eccentric part of every the exercise. This as well as the imperative progression to higher weights, whence the prescribed number of 8-12 reps per set could be performed is unfortunately overlooked by many recreational trainees - with disappointing consequences in the form of inferior or even no size and strength gains, by the way... but I am digressing, let's rather take a look at the already mentioned, unexpectedly superior strength size gains in the long(er) rest interval group (Figure 3).
Figure 3: Changes in muscle thickness and corresponding effect sizes; * denotes significant pre- vs. post-changes, # denotes significant inter-group differences; overall it is obvious that there's a long(er) rest advantage (Schoenfeld. 2015).
As the single "#" in Figure 3 tells you, the inter-group differences and thus the advantage of the long(er) rest intervals was statistically significant only for the quads, though. If we also take into account the lack of statistically significant effects on the sleeve sizes (biceps and triceps) in the short rest interval group, as well as the obvious differences in effect sizes (Figure 3, right), there's yet little doubt that the hypothesis that shorter rest intervals yield greater size increases must be considered falsified - at least under the given experimental conditions (trained subjects, three full-body workouts per week, standard hypertrophy set and rep-ranges, etc.).
So what's the verdict, then? At first sight it would appear as if the study at hand would totally refute the idea that shorter rest intervals, or I should clarify, rest intervals that are as short as 60s (*) should have a place in your training regimen altogether (*Schoenfeld, et al. rightly point out that Ahtiainen's result suggest that even 120s could have been enough time to rest - it is thus important to give precise recommendations for rest intervals, not something as arbitrary "short" vs. "long"). We should not forget, though, that even a thoroughly conducted study like the one at hand has its limits and definite conclusions should not be drawn hastily based on a single study result - even if it is, as in this case, corroborated by the results of Buresh et al (2009).

Figure 4: The total training volume in the long(er) rest period group (3 vs. 1 minutes of rest) was on average 15% higher. Due to the relatively high inter-individual differences and the relatively low number of participants (N=21) a statistically significant correlation between the weight lifted per week (total volume in kg as in the figure) and the surprisingly superior gains in the 3-min-rest group could not be established (based on Schoenfeld. 2015).
With that being said, a secondary outcome of the study provides a reasonable explanation for why both, the strength and the size gains benefited from long(er) rest intervals: The total training volume I've plotted in Figure 4. As Schoenfeld et al. point out, the latter has previously been suspected to mediate the effects of inter-set rest on strength and hypertrophy on total training volume and strength (Henselmans. 2014). A correlation between the visible differences in training load (see Figure 4) and the magnitude of training adaptations, however, could not be found in the study at hand. As the authors highlight, the reason for this lack of statistical significant correlations may yet be a simple lack of statistical power, so that one "cannot rule out the possibility that the greater training load achieved by the longer rest period group was responsible for the greater training adaptations" (Schoenfeld. 2015 | Buresh et al. found such an effect for the upper, yet not for the lower body).

Personally, I tend to believe that, with a higher number of subjects, a correlation between the total training volume that was on average 15% higher in the 3 vs. 1 minute rest group could have been established. This, in turn, would support the notion that long(er) rest periods - maybe, as Schoenfeld et al. suggest based on the data from Ahtiainen's study, at least 120s - are necessary to maximize the total training volume and thus the overall = strength and hypertrophy response to workouts. Whether that is true for all types of workouts (e.g. split- vs. full-body), all subject groups (e.g. people who are used to short rest periods vs. those who are not) as well as special athletic requirements (e.g. power vs. strength & hypertrophy) will have to be determined in future studies, however | Comment on Facebook!
References:
  • Ahtiainen, Juha P., et al. "Short vs. long rest period between the sets in hypertrophic resistance training: influence on muscle strength, size, and hormonal adaptations in trained men." The Journal of Strength & Conditioning Research 19.3 (2005): 572-582.
  • Buresh, Robert, Kris Berg, and Jeffrey French. "The effect of resistive exercise rest interval on hormonal response, strength, and hypertrophy with training." The Journal of Strength & Conditioning Research 23.1 (2009): 62-71.
  • Henselmans, Menno, and Brad J. Schoenfeld. "The Effect of Inter-Set Rest Intervals on Resistance Exercise-Induced Muscle Hypertrophy." Sports Medicine 44.12 (2014): 1635-1643.
  • Schoenfeld, Brad J. "The mechanisms of muscle hypertrophy and their application to resistance training." The Journal of Strength & Conditioning Research 24.10 (2010): 2857-2872.
  • Schoenfeld, et al. "Longer inter-set rest periods enhance muscle strength and hypertrophy in resistance trained men." Journal of Strength and Conditioning Research (2015): Publish Ahead of Print.
  • Villanueva, Matthew G., Christianne Joy Lane, and E. Todd Schroeder. "Short rest interval lengths between sets optimally enhance body composition and performance with 8 weeks of strength resistance training in older men." European journal of applied physiology (2014): 1-14.
  • Willardson, Jeffrey M. "A brief review: factors affecting the length of the rest interval between resistance exercise sets." The Journal of Strength & Conditioning Research 20.4 (2006): 978-984.
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No Additional Gains With PWO Protein + Leucine Shakes in Rookies? Why the "Shocking" Results of a Recent Study Don't Mean That They, Let Alone You, Cannot Benefit at All

You can't expect wonders from protein supplements... no matter, when you take them, by the way. But I wouldn't mark them down as useless - even for rookies - based on the study at hand.
When I look at the 14 year old kids who need an written permission from their mum to train at the gym buying protein tons o powders and dozens of bars from the vending machines at the gym, even I have to shake my head. Not necessarily, because they're buying those products, but rather about what they tell each other about the effects: "Yo,... recently I ate this protein bar right after my workout and I felt swole for day..."

Now, you know that I am into supplement psychology and have no doubt about the fact that said kiddo felt swole all day, but we all know that protein is in no way a supplement the muscle-building and strength advantages of which will show within hours. And with that, I have a perfect transition to today's topic: The recent study from University of Central Florida in Orlando (Boone. 2015).
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You will probably remember the most striking result of this study in which Carleigh H. Boone and colleagues "examine[d] the short-term effect of resistance training with and without protein supplementation on changes in muscle morphology and strength in untrained young men" (Boone. 2015).

Some of you may now be wondering: Why on earth do they even test that? We have ample evidence that protein supplements are powerful muscle builders! And you are right, but as it is the case for (almost) every research question in exercise science, even the highly acclaimed benefits of pre- or post-workout protein supplementation are somewhat contested. There's the evidence that supports the benefits of peri-workout protein supplementation on exercise induced muscle gains. Unfortunately, many of these studies may have been over-interpreted. Why?

FSR ≠ more muscle. Just because the post-exercise fractional protein synthesis increases this does not mean that you will gain more muscle in the long term. Check out this SV Classic to learn why!
Well, many of the often-cited studies in rookies, like Biolo et al. (1995), Phillips et al. (1997), or Tipton et al. (1999), measured increases in acute muscle protein synthesis and markers of anabolism. Due to their short design, however, increased long-term gains could only be predict, but not detect. In my 2014 article "Study Confirms: Acute Post-Exercise Myofibrillar Protein Synthesis Is Not Correlated with Resistance Training-Induced Muscle Hypertrophy in Young Men" (read more) you have learned, though, that the assumption that significant increases in post-workout muscle protein synthesis will necessary lead to significant augmented increases in muscle size in the long run is logical. but futile.

There are studies where this is the case, e.g. Coburn et al. (2006); Hulmi et al. (2010); Walker et al. (2009); Willoughby et al. (2007) - in both, trained and untrained individuals, but as Boone et al. rightly point out, there are also studies by Antonio et al. (2000) or Hoffman et al. (2009) in which protein or amino acid supplements had no beneficial effects on muscle or strength gains in either or trained and untrained individuals. The randomized, double-blinded study Boone et al. (2015) conducted is thus by no means superfluous. It is yet no perfect either, because with a length of only our week, the scientists picked exactly the time-frame in which things, or I should say "gains" happen faster than you can measure them. Furthermore, the use o a lower body unilateral resistance training makes it very difficult to infer the effects of protein supplementation on (a) muscle morphology [cross-sectional area, muscle thickness, pennation angle], (b) lower body power output, (c) maximal dynamic strength, and (d) maximal isometric strength in other muscle groups and in response to an overall metabolically more demanding workout.
Protein Timing Does Matter! Yet Only in Trained Men. More Than 2x Higher Relative Protein Retention W/ Immedi-ate vs. 6h Post Whey Consumption in Bodybuilders vs. Rookies | read more
Why does trained vs. untrained matter? We know that the growth stimulus in response to exercise and the unused growth potential are both maximal in untrained individuals. As far as strength is concerned, they have the additional advantage of making rapid gains by learning to master the exercises properly and neurological adaptations which are for the most part responsible for the rapid strength increases in the first months of resistance training. It is thus only logical that there's evidence that rookies do in fact gain "no matter what", while advanced trainees will have to up their game by advanced training techniques, protein supplementation and as I pointed out in a 2014 article, here at the waredownloadsoft even protein timing, which appear to be pretty useless for the people who have just been picking up the bar- and dumbbells.
Since the scientists have concise dietary records (Figure 1) and used a supplementation regimen that included not just 17g of whey protein concentrate, but also 3g of cholostrum and an extra 2g of leucine (PLA was an iso-caloric amount of resistant maltodextrin) to trigger a maximal increase in protein synthesis when the shake was consumed ad-libitum (in this case whenever the subjects wanted) on non-training days and immediately after the training sessions on training days, the data is still worth discussing (also because I already mentioned it on Facebook and I don't want you to over-estimate the overall significance of the study).
Figure 1: Overview of the macronutrient (in g) composition of the subjects' diets at baseline and during the study (the value for protein include the amount of protein from the protein shake in the PRO group | Boone. 2015).
As you can see in Figure 1 the protein intake of the twenty untrained young men who were randomly assigned to either a resistance training + protein (PRO; 21.4 ± 1.9y, 75.3 ± 15.7kg) or resistance training + placebo (PLA; 22.9 ± 3.1y, 76.8 ± 14.4kg) group was almost identical at baseline (1.2g/kg) and increased from 1.2 to 1.3g/kg in the protein group (+18.2%) while it dropped to 1.0g/kg body weight in the placebo group (-17.1%).

No such differences existed with respect to the supervised resistance training programs which started with five minutes of non-fatiguing aerobic activity on a cycle ergometer at a self-selected resistance and cadence. This was followed by a specific warm-up consisting of body weight squats, alternating lunges, walking knee hugs, and glute kicks (10 each). The subsequent, actual training sessions is described as follows.
"Each training session consisted of unilateral countermovement jumps (CMJ), leg press (LP), and leg extension (LE) using the dominant leg. CMJ were performed for a total of three sets of 8 repetitions with maximal effort. The LP and LE exercises were performed for a total of three sets of 8-10 repetitions at 80% of the participant’s previously determined 1RM with 90 seconds allotted between sets and exercises. If a participant was unable to perform the minimum amount of repetitions during the first or second sets of LP or LE, weight was decreased to an intensity deemed appropriate by a certified trainer. Consequently, if the participant was able to perform all repetitions with proper form and minimal strain, weights were progressively increased during the subsequent training session at the certified trainer’s discretion" (Boone. 2015).
After twelve of these training sessions, which took place at the university’s Strength and Conditioning Lab, on three nonconsecutive days per week.
Why would it have been nice to know when the size and power gains were measured? The answer is "the pump", ... well, not exactly. It's rather the cell swelling that occurs in the hours and days after a workout. The latter may have increased the overall impression of size gains and thus reduced the significance of potential differences if there was not a interval of at least 5 better 7 days between the last workout and the measurement of the muscle sizes. Bullocks?
Working out leads to increases in water content (left) corresponding increases in muscle "size" (right) at the 10 & 20 cm measuring points of the the quads; all values expressed as relative changes (%) vs. baseline | more
No, reread my 2014 article "Cell Swelling Keeps Muscles "Pumped" For More Than 52h. Size Increases of Up to 16% After a Single Leg Workout! Plus: Changes in Tendon Water & Collagen Content" (read the full SV-Classic article)- that the data will be distorted is a proven fact, with just one study investigating the extent, though, we cannot tell if the data will always be 16% off and the difference thus practically relevant.

Another reason why I was specifically looking for information on the interval between the last training and testing sessions was the lack of increases in peak and mean power (the changes in Figure 2 are all non-significant). If we know when the testing was done, one may be able to tell if this was a result of residual fatigue or if there were in fact no power gains in any of the two groups.
I've plotted the size, strength and power gains, which were assessed on an unfortunately non-disclosed time (see box above for elaborations) in Figure 2:
Figure 2: While even the small size gain in the rect. femoris in the PRO group was stat. sign. compared to the pre-value, none of the visible differences between PRO and PLA reached stat. significance and the mean and peak power did not even change at all... well, statistically speaking (see box above for a discussion why this may be the case | Boon. 2015)
Now, it's not like there were no gains, the cross-sectional area and muscle thickness increased significantly, but the scientists observed neither statistically nor practically significant (mostly due to the large inter-individual differences, e.g. a standard deviation of +/-12% for the CSA increase of the Rectus Femoris in the PRO group).
Table 1: Changes in maximal dynamic, isometric, and specific strength following training - Values are means ± SD. * Significant change from Pre to Post (p < 0.05 | Boone. 2015).
Since the strength data in Table 1 is similarly inconclusive, there is little doubt that is - for the study at hand - 100% warranted to say that while "the current findings suggest that short-term resistance training resulted in significant increases in muscle strength and size in untrained young men", they also suggest that "[t]he addition of a post-exercise protein supplement used in the current investigation was not sufficient to enhance the effect of resistance training" (Boone. 2015).
Polydextrose and Resistant Malto-dextrin May be Useful Dieting Aids, But are They good Placebos?
So what? Don't dumb your protein supplements, yet ;-) Aside from the previously mentioned study duration and the unrealistic training regimen, there is another thing I would like to highlight to substantiate my previously voiced warning that you should not overestimate the practical significance of the study at hand: The "placebo" supplement. Usually you'll see that protein is tested against regular maltodextrin. In the study at hand, however, it was tested against resistant maltodextrin of which you, as a waredownloadsoft reader know that it has been shown to significantly curb the appetite in humans and rodents. More recently, Hira et al. (2015) calculated that only 10g of these resistant starches per day may be able to reduce the energy intake by a whopping 7%.

This puts the -9.4% reduction in energy intake in the placebo group into an altogether different light and makes me question whether the placebo was as inactive as it should be. I have to admit, though, that eventually a lower total protein intake and a lower total energy intake as it was observed in the PLA group at identical study outcomes would even substantiate the claim that additional protein is useless in rookies, but who knows which other unknown effects the 20g of the resistant maltodextrin "placebo" had ;-)

Enough of the speculations, though. Let's stick to the facts: (1) Very few of you will be in the first four weeks of their resistance training "career", (2) even fewer of you will train only one leg, and I'd hope that (3) none of you plan to train for only the next for weeks only. So, that alone would make me want to repeat the scientists' own suggestion that "more research needs to be done" while telling you to (a) keep the results of this study in mind and file them under "protein is no wonder drug", while (b) keep taking your protein supplements. There's good enough evidence to say that >90% of you are going to benefit in one area or another - and always remember whey is more than a muscle builder (learn more) | Comment on Facebook!
References:
  • Antonio, Jose, et al. "Effects of exercise training and amino-acid supplementation on body composition and physical performance in untrained women." Nutrition 16.11 (2000): 1043-1046.
  • Biolo, Gianni, et al. "Increased rates of muscle protein turnover and amino acid transport after resistance exercise in humans." American Journal of Physiology-Endocrinology And Metabolism 268.3 (1995): E514-E520.
  • Coburn, Jared W., et al. "Effects of leucine and whey protein supplementation during eight weeks of unilateral resistance training." The Journal of Strength & Conditioning Research 20.2 (2006): 284-291.
  • Boone, Carleigh H., et al. "Muscle strength and hypertrophy occur independently of protein supplementation during short-term resistance training in untrained men." Applied Physiology, Nutrition, and Metabolism ja.
  • Hira, Tohru, et al. "Resistant maltodextrin promotes fasting glucagon-like peptide-1 secretion and production together with glucose tolerance in rats." British Journal of Nutrition (2015): 1-9.
  • Hoffman, Jay R., et al. "Effect of protein-supplement timing on strength, power, and body-composition changes in resistance-trained men." International journal of sport nutrition 19.2 (2009): 172.
  • Hulmi, Juha J., Christopher M. Lockwood, and Jeffrey R. Stout. "Review Effect of protein/essential amino acids and resistance training on skeletal muscle hypertrophy: A case for whey protein." Nutrition & metabolism 7 (2010): 51.
  • Phillips, Stuart M., et al. "Mixed muscle protein synthesis and breakdown after resistance exercise in humans." American Journal of Physiology-Endocrinology And Metabolism 273.1 (1997): E99-E107.
  • Walker, Thomas B., Et Al. The Influence Of 8-Weeks Of Whey Protein And Leucine Supplementation On Physical And Cognitive Performance. Air Force Research Lab Brooks Afb Tx Human Effectiveness Directorate, 2009.
  • Willoughby, D. S., J. R. Stout, and C. D. Wilborn. "Effects of resistance training and protein plus amino acid supplementation on muscle anabolism, mass, and strength." Amino acids 32.4 (2007): 467-477.
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Chains & Bands Can Double Your 1RM Strength Gains on the Bench and in the Squat Rack, Meta-Analysis Shows

Dude, it won't suffice to just bring your chains to the gym to show them off, you will also have to attach them to the barbell before squatting and benching to see results... and bro, the science on the benefits of elastic bands is much more solid - even though they are not as "cool"!
I've written about the use of bands and chains in previous waredownloadsoft articles, but Miguel A. Soria-Gila recent paper is the first meta-analysis that aggregates the available data to answer the important question, whether the use of "variable resistance" training (VRT), as the use of bands and chains is usually referred to in the literature, is generally advisable, or if the existing positive results are nothing but outliers.

Now, from the headline of today's waredownloadsoft article you already know that Sotia-Gila's analysis yielded positive results, or as the authors have it: " Long-term VRT training using chains or elastic bands attached to the barbell emerged as an effective evidence-based method of improving maximal strength both in athletes with different sports backgrounds and untrained subjects."
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What is particularly interesting, though, is whether the statistically significant benefits are practically relevant enough for you to consider bringing your chains and/or resistance bands to the gym.
Figure 1: Relative strength increase in bench press (BP), back squat (BSQ), leg press (LP) and squat (SQ) in response to regular and variable resistance training; if not indicated otherwise, the variable resistance training was done with bands, only the study by Ghigarelli, et al. compared bands to chains (Soria-Gila. 2015).
To answer this question we need both, the relative and absolute strength increases in both, the variable resistance training (VRT) and control groups of the four pertinent studies in the meta-analysis - data I've plotted for you in Figure 1 and 2.
Figure 2: Absolute increase in 1-RM strength (all values in kg) in the respective exercises (see Figure 1 for abbreviations) in the seven 7-week plus studies that were part of the meta-analysis (Soria-Gila. 2015).
In five of the studies (indexed with "(T)" in Figure 1) the subjects were trained individuals, in the studies by Anderson (basketball and hockey players + wrestlers), Cronin and McCurdy (baseball, Division I) the subjects actually had ~3 or even more years of training experience. The results of these studies may thus be of particular interest for the average waredownloadsoft reader of whom I know that he / she is not a total foreigner to gym. If we assume that they / you would see the same benfits, the extra-increases on the bench and in the squat would be:
  • An extra 5% increase in 1RM and thus 2x greater strength gains on the bench.
  • An extra 11% increase in 1RM and thus 2.6x greater strength gains for squats.
In relative terms the benefits you may achieve after only 10-13 weeks are thus quite impressive. But can the same be said for the absolute extra-gains? Soria-Gila et al. report an extra strength gain of 5.03 kg (95% confidence interval: 2.26–7.80 kg) for all studies and all exercises. If we, again, consider only the bench press and the squat and eliminate the studies with untrained participants, the absolute values are much smaller: 1.8 kg and 2.7 kg, respectively.
Are you looking for more ways to maximize your strength gains? Find out if training to failure or modifying your rest times can help in this waredownloadsoft article.
Variable resistance training for explosive gains? In relative terms, the effects are huge. Two-fold larger increases in 1-RM strength in trained subjects speak for themselves. The absolute strength gains, on the other hand, are - and that's typical for people who have been training for several years - relatively small. Accordingly, you should not expect to start gaining strength like a rookie again, when you incorporate bands (which are better researched than chains) in your training regimen. What you can expect, though, is that your progress will accelerate significantly. For the next 2-3 months this would mean that you may be able to add 4 kg to your bench instead of just 2 kg. That's not exactly earth-shatteringly much, but it's still a 100% increase in 1-RM strength and in my humble opinion worth the effort... no? | Comment on Facebook!
References:

  • Anderson, Corey E., Gary A. Sforzo, and John A. Sigg. "The effects of combining elastic and free weight resistance on strength and power in athletes." The Journal of Strength & Conditioning Research 22.2 (2008): 567-574.
  • Bellar, David M., et al. "The effects of combined elastic-and free-weight tension vs. free-weight tension on one-repetition maximum strength in the bench press." The Journal of Strength & Conditioning Research 25.2 (2011): 459-463.
  • Cronin, John, Peter Mcnair, and Robert Marshall. "The effects of bungy weight training on muscle function and functional performance." Journal of sports sciences 21.1 (2003): 59-71.
  • Ghigiarelli, Jamie J., et al. "The effects of a 7-week heavy elastic band and weight chain program on upper-body strength and upper-body power in a sample of division 1-AA football players." The Journal of Strength & Conditioning Research 23.3 (2009): 756-764.
  • McCurdy, Kevin, et al. "Comparison of chain-and plate-loaded bench press training on strength, joint pain, and muscle soreness in Division II baseball players." The Journal of Strength & Conditioning Research 23.1 (2009): 187-195.
  • Rhea, Matthew R., Joseph G. Kenn, and Bryan M. Dermody. "Alterations in speed of squat movement and the use of accommodated resistance among college athletes training for power." The Journal of Strength & Conditioning Research 23.9 (2009): 2645-2650.
  • Shoepe, Todd, et al. "The effects of 24 weeks of resistance training with simultaneous elastic and free weight loading on muscular performance of novice lifters." Journal of human kinetics 29 (2011): 93-106.
  • Soria-gila, Miguel A., et al. "Effects of variable resistance training on maximal strength: a meta-analysis." Journal Of Strength And Conditioning Research/National Strength & Conditioning Association (2015): Accepted article.
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